The present study investigates the content of microbial flora in the infected canals and periodontal pockets of the teeth with the endo-periodontal syndrome, for establishing the frequency of the bacterial associations thus
determined. Materials and method. The content of 25 radicular canals and of 20 periodontal sites, with pockets between 3-5 mm, taken over from 20 patients, clinically and radiographically diagnosed with the endo-periodontal syndrome, was evaluated by microbial analysis. Identification of the isolated bacterial species considered: the growing aspect of the colonies (colony morphology) and also the morpho-tinctorial aspect in some isolated colonies.
Results and discussion. The 25 endodontic microbial samples contained cultivable microorganisms. The average number of CFU ml-1 was 8×104 per sample, while the number of species per canal varied between 4 and 7 (with a mean value of 5.1). The microorganisms from the periodontal pockets were all positive for the anaerobic flora. Out of their total number, 50 bacterial species were identified, 4 strains remaining unidentified.
Conclusions. The results obtained show that the endodontic pathogenic agents do not occur randomly, being present, instead, in specific combinations, which may contribute to the manifestation of some clinical signs and symptoms. The diseases of the marginal periodontium are undoubtedly related to the existence of the Gram-negative microbial species present at subgingival level. The microbiological tests provide important data for a correct selection of the antibiotic treatment recommended by the antibiogram.