Introduction: In dentistry, diabetic wounds can lead to necrosis. Alginate-chitosan scaffolds with silver nanoparticles can aid healing due to their anti-inflammatory and anti-bacterial properties. Aim: To produce biocompatible silver nanoparticles loaded alginate chitosan scaffolds for dental applications and to characterize their properties and determine their biocompatibility.Materials and methods: Biosynthesis of AgNPs was carried out using a plant extract of Evolvulus alsinoides loaded into the alginate-chitosan scaffold. AgNPs were characterized using UV spectrophotometer and FT-IR. The morphology of AgNPs loaded alginate-chitosan scaffold was examined using SEM. Annexin V PI apoptotic was used for checking its biocompatibility. Results and discussion: The UV-Vis spectrum gave a strong peak of 417 nm, confirming AgNPs formation. Similarly, FT-IR has strong absorption bands at 3217, 1598, 1406, 1027, 945, and 817 cm−1, with wavelengths ranging from 4000-500 cm-1. SEM analysis revealed the dispersion of AgNPs in the porous alginate-chitosan scaffold. Our results suggest that biosynthesized silver nanoparticles loaded with alginate-chitosan scaffold possess greater biocompatibility towards PBMCs, as evaluated by Annexin V – PI assay. Conclusions: Silver nanoparticles were successfully synthesized by the biological method using leaf extract of Evolvulus alsinoides, and reported as biocompatible using flow cytometry. However, a more detailed study should be done before using it as therapeutics for diabetic wound healing in dentistry.
Keywords:- biocompatibility.
- diabetic wound healing
- Evolvulus alsinoides
- silver nanoparticles