Introduction: Localized inflammatory lesions in one area of the body may affect other distant organs through various modes of transmission, thus initiating secondary inflammatory infections. Periodontal disease (PD) and inflammatory bowel disease (IBD) have been shown to coexist. Recent studies and systematic reviews have focused on evidence-based statistics of the prevalence and clinical manifestations of both diseases, but discussions about the microbial etiological correlation between periodontitis and intestinal inflammation are scarce. This study aims at assessing that the gene-gene interactome of periodontal disease and inflammatory bowel disease regulates RHO GTPase and the actin cytoskeleton.Materials and methods: Differentially expressed genes (DEGs) of periodontitis and inflammatory bowel disease were identified from the datasets retrieved from the Gene Expression Omnibus database. GeneMANIA was used to visualize molecular interaction networks. By ranking the scores of each node of molecular interaction, the important nodes of protein interactions were obtained. The hub genes were ranked and those with degree ≥ 20 were selected. A network of genes and their co-expression genes was analyzed. Gene ontology of the corresponding genes were also assessed.Results and discussion: Members of the Rho family of small guanosine triphosphatases have emerged as key regulators of the actin cytoskeleton. The RhoA family regulates actin cytoskeletal dynamics that many bacterial pathogens modify in order to invade host cells for their survival. This signaling pathway interaction provides evidence to be common in periodontal disease and inflammatory bowel disease. Conclusions: The top five ranked hub genes involved in the interactome are ESYT2, KLK8, ARHGEF5, CTSV, SPINK5.
Keywords:- differentially expressed genes
- inflammatory bowel disease
- interactome
- periodontitis