Introduction: In the field of dentistry, natural polymers are being investigated for their potential in tissue engineering. Alginate and chitosan are commonly used scaffolds due to their biodegradable nature, compatibility, and structure similarv to that of the extracellular matrix. Quercetin, a flavonoid with various biological properties, is combined with these scaffolds to enhance tissue repair and regeneration, ultimately improving wound healing in dental applications.Aim: To incorporate quercetin into alginate-chitosan scaffolds and evaluate their regenerative potential in dentistry through characterization and assessment of biocompatibility. Materials and methods: Quercetin was impregnated onto the alginate-chitosan scaffold, then characterized using Fourier Transform-InfraRed (FT-IR) and Scanning Electron Microscopy (SEM). Annexin V PI apoptotic assay and Hemolytic assay were done for checking its biocompatibility. Results and discussion: FT-IR has strong absorption bands at 3305, 1595, 1408, 1290, 941, and 819 cm−1, with wavelengths ranging from 4,000-500 cm-1. SEM analysis confirmed quercetin impregnation onto the alginate-chitosan scaffold. Based on our findings, the combination of quercetin and alginate-chitosan scaffolds exhibits improved biocompatibility towards Peripheral blood mononuclear cells (PBMC), as determined by Annexin V – PI assay and hemolytic assay, indicating potential for dental regenerative applications.Conclusions: Quercetin impregnated onto an alginate-chitosan scaffold is reported to be biocompatible using flow cytometry and hemolytic assay. Further extensive investigation is necessary before considering their use in regenerative dentistry.Keywords:
- regenerative dentistry
- tissue engineering.