Introduction. Fractures of the lower jaw are one of the most common injuries in the head and neck. Bone regeneration is a key step in the fracture healing process and plays an important role in restoring jaw function. However, the presence of endogenous intoxication can complicate the healing process and lead to a decreased efficiency of the regenerative process. In this article, we examine the relationship between the regenerative process of bone tissue in mandibular fractures and the level of endogenous intoxication. We investigate the mechanisms of bone regeneration and study the effect of endogenous intoxication on the healing process. Our research will help better understand the healing processes of mandibular fractures and develop more effective treatments.The aim of the study was to establish the effects of chronic endogenous intoxication on both intact bone and the process of reparative osteogenesis in mandibular fractures.Materials and methods. Regularities of morphofunctional transformations in bone tissue were studied during spontaneous healing of mandibular fractures in rats against the background of chronic endogenous intoxication after 30, 60 and 90 days. Simulation of chronic endogenous intoxication was performed by repeated administration of tetrachlormethane and bacterial lipopolysaccharide doses. Determination of medium molecular weight substances, as well as their oligopeptide fraction in blood serum were used as integral laboratory and biochemical indicators of ET development. Concentrations of products of free radical oxidation of lipids were also determined in the blood plasma of rats. Monoclonal antibodies to the proliferating cell nuclear antigen PCNA were used to detect proliferative activity in the regenerate and surrounding bone tissue. The morphometric study was performed in accordance with the principles of systemic quantitative analysis.Results. The formation of an osteoregenerate at mandibular fracture in rats against the background of chronic endotoxicosis is accompanied by a decreased growth of the volume fraction of bone tissue and stromal-vascular component which, according to correlation analysis, is associated with the level of medium molecular weight substances and malonic dialdehyde in blood plasma. There is a strong positive correlation between the volume fraction of the regenerate vessels and the level of malonic dialdehyde (r=0.671-0.692). Negative correlations were detected for the numerical̆ density of osteoblasts and osteocytes (r=-0.622) and positive correlations for osteoclasts (r=0.654-0.873). Conclusions. The study provided a lot of morphological evidence of the effect of chronic endogenous intoxication on both intact bone and the process of reparative osteogenesis after mandibular fractures.
Keywords:- chronic endogenous intoxication
- mandibular fractures
- reparative osteogenesis