Introduction: F. Benghalensis is known to have medicinal effects and is beneficial in treating mouth ulcers. A. Indica is rich in antioxidants and helps boost the immune response in gum and tissues of the mouth. Neem offers a good remedy for curing mouth ulcers, tooth decay and acts as a pain reliever. M. Piperita possesses anti-inflammatory properties that promote oral hygiene. Aim: To prepare a mouth wash formulation containing F. Benghalensis, A. Indica, M.Piperita and to determine the antioxidant and anti-inflammatory effects of mouthwash formulations containing F.Bbenghalensis, A. Indica, M. Piperita. Materials and methods: In the present study, preparation of herbal mouthwash formulation was carried out and the antioxidant and anti-inflammatory activity of the mouthwash was assessed by the DPPH Assay method and Hydroxyl Radical Scavenging Assay method. The anti-inflamnatory activity of the mouthwash was assessed using albumin denaturation assay method and egg albumin denaturation assay method.Results: The antioxidant and anti-inflammatory activity was higher at low concentrations while, at concentrations of 10-30 μl, the antioxidant activity of the prepared mouthwash was similar to that of the standard mouthwash. The anti-inflammatory activity was the highest at 20 μl concentration. Discussion: Ficus benghalensis, Azadirachta indica and Mentha piperita extracts have many medicinal properties. Our study has evaluated the combination of these three extracts in a mouthwash formulation and has found positive results.Conclusions: The mouthwash formulation had better antioxidant and anti-inflammatory activity at lower concentrations.
Keywords:- A. indica
- antimicrobial activity
- cytotoxicity
- F. benghalensis
- M. piperita